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Cambridge Cambridge International A-Level Science

Cambridge Cambridge International A-Level Science Learning Path

Detailed syllabus unit mapping, custom lesson objectives, required laboratory practical guides, and assessment MCQ checklists.

Advanced A2 syllabus. Covers gravitational fields, PCR gene technologies, electrochemistry thermodynamics, and biochemistry.

Lesson Plans & Objectives

Polymerase Chain Reaction (PCR)60 min

Explain denaturation, annealing, and extension temperature cycles.
Describe the role of Taq polymerase enzyme.

Electrophoresis and Plasmids60 min

Outline gel separation of DNA fragments based on charge.
Explain plasmid vectors in transformation.

Suggested Practical Activities

Extracting genomic DNA from strawberries and running mock agarose gel dye separations

Revision Notes

PCR steps: Denaturation (95°C), Annealing (55-60°C), Extension (72°C).
DNA is negatively charged (due to phosphate groups) and migrates towards the positive anode in an electric field.